One-cell ova were recovered from superovulated rabbit and pig donors. For microinjection a DNA fragment consisting of the structural gene of the human growth hormone and the promotor region of the mouse methallothionein gene was used.
After transfer of injected eggs 37 (9.6 %) offspring were born in rabbits and 15 (3.4 %) in pigs. 5 rabbits and 1 piglet were found to be transgenic by dot hybridisation and Southern blotting.

G. Brem, B. Brenig, H. M Goodman, R. C Selden, F. Graf, B. Kruff, K. Springmann, J. Hondele, J. Meyer, E. L Winnacker, H. Krausslich

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume XII. Biotechnology, selection experiments, parameter estimation, design of breeding systems, management of genetic resources., , 45–50, 1986
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