The distribution and abundance of hypervariable DNA markers in the chicken genome was examined using multilocus DNA fingerprinting. Minisatellite alleles at loci detected using the Jeffreys probe3 33.6 and 33.15 in a family containing two parents and thirteen offspring were found to segregate independently, and band transmission followed mendelian expectation. A low level of cosegregation (6.2%) and apparent allelism (13.8%) was found, and a higher than previously described level of homozygosity (27%) was attributed to the fact that the parents originated from two inbred lines. Locus-specific probes were isolated from two chicken genomic DNA libraries and tested on a panel of four unrelated individuals. Early results suggest that these probes detect loci with observed heterozygosity values of 80% to 100%. It is proposed to use the locus-specific probes in strain identification, genome mapping (including resolution of quantitative trait loci) and marker assisted selection.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume XIII. Plenary lectures, molecular genetics and mapping, selection, prediction and estimation., , 139–142, 1990
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