Abstract

The aim of the research was to find Restriction Fragment Length Polymorphisms (RFLPs) at the bovine ds^-, Cts2-. and j9-casein loci. Milk and DNA samples from 98 cows belonging to six breeds (Italian Friesian, Reggio, Modena, Podolian Type, Italian Brown, and Jersey) were considered. DNA samples were digested with the following enzymes: BamHI, BglTI, EcoRI, EcoRV, Hindlll, PstI, PvuII, and TaqI. Three endonucleases (TaqI, B glll, and EcoRI) evidence RFLPs at the dsi -Cn locus. With the exception of few recombinants in the Reggio and Modena breeds, Bglll and EcoRI polymorphic fragments correspond to B and C alleles of Ctsi -casein. A RFLP was found at the <Xs2 -Cn locus with TaqI endonuclease and no RFLP was found at the ft-Cn locus.
 

A. Rando, P. di Gregorio, R. Davoli, S. Dall'Olio, P. Masina, V. Russo

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume XIV. Dairy cattle genetics and breeding, adaptation and conservation., , 241–244, 1990
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