Abstract

Preliminary results from an ongoing investigation of restriction fragment length polymorphism (RFLP) at genes in the growth hormone axis are presented. Twelve heifers of Red Danish Cattle from two lines selected for high and low fat yield were screened for restriction fragment length polymorphisms around the growth hormone gene and the insulin-like growth factor-I gene. A polymorphism at the growth hormone gene consistent with an insertion/deletion of 0.9 Kb was found in the two lines. The frequency of each of the alleles was not different between lines. No RFLPs were found for the insulin-like growth factor-I gene. Screening of pigs from Landrace, Yorkshire, Duroc and Hampshire identified a RFLP consistent with a point mutation at the growth hormone gene with the Dral enzyme. Investigation of 8-19 unrelated pigs from each of the four breeds revealed significant differences in the frequencies of the two alleles between breeds.
 

S. Hoj, N. J Larsen, V. H Nielsen

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume XIII. Plenary lectures, molecular genetics and mapping, selection, prediction and estimation., , 151–154, 1990
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