Ten minisatellite sequences were assessed for their ability to detect restriction fragment length polymorphism (RFLP) markers. The sequences detected multiple marker loci with the mean number of variable bands for each sequence ranging from 2.4 to 12.2 per animal. However there was extensive band sharing between some of the minisatellite probes thereby reducing the number of independent loci analysed. In situ hybridisation of one minisatellite probe revealed that 33% of the loci were telomeric. However the remaining loci were randomly scattered across autosomes. Despite these limitations, the multi-locus minisatellite approach to linkage analysis was considered the most effective currently available for sheep.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume XIII. Plenary lectures, molecular genetics and mapping, selection, prediction and estimation., , 129–132, 1990
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