Abstract

A swine chromosome 6 specific library was generated using size fractionated DNA isolated from chromosomes sorted by flow cytometry. Procedures have been established in our laboratory to reproducibly prepare high quality chromosomes from PHA stimulated swine peripheral blood lymphocytes and to sort individual chromosomes after staining with Hoechst 33258 and chromomydn A3. DNA from 1,000 isolated chromosome 6 was then Sau3A digested cloned into pBluescript SKII+, and PCR amplified; 300-600bp DNA fragments were isolated from the amplified DNA and cloned to produce the chromosome 6 specific library. Our chromosome 6 library has already yielded 17 clones containing (CA)n repeats and is now being screened for more (CA)n microsatellite repeats as well as other repeat sequences. New microsatellite markers on chromosome 6 will be used to develop a more detailed map of the region where the genes that encode positive carcass traits and Porcine Stress Syndrome (PSS) are found

D. R Grimm, T. Goldman, R. Holley, Joan K Lunney

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 21. Gene mapping; polymorphisms; disease genetic markers; marker assisted selection; gene expression; transgenes; non-convention, , 75–78, 1994
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