The random amplified polymorphic DIMA (RAPD) assay was evaluated as a means of identifying genetic markers in sheep. DNA samples from an AgResearch International Mapping Pedigree, consisting of maternal and paternal grandparents, sire, dam, and twelve full-sib progeny, were screened with 130 10-mer oligonucleotide primers of arbitrary sequence. A total of 96 polymorphisms were identified between the sire and dam, and 56% (54/96) of these markers segregated in the progeny. Screening of the grandparental samples confirmed the origin of the segregating parental polymorphisms and the consistency and reproducibility of the assay. Three candidate Y-linked markers were identified, and their chromosomal assignment was confirmed by screening four other AgResearch pedigrees. These results demonstrate that the RAPD assay is very effective for identifying genetic markers in sheep. We are continuing to screen the AgResearch International Mapping Pedigrees, with the objective of contributing RAPD markers to the sheep genetic map.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 21. Gene mapping; polymorphisms; disease genetic markers; marker assisted selection; gene expression; transgenes; non-convention, , 133–136, 1994
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