Embryonic stem cell technology has emerged from the dem onstration that in vitro cultured cells could re-populate the germ line to a facile system for the analysis of gene fuction. In this report some of the key technical advances that facilitated the development of this technology in the m ouse will be reviewed. The types of genetic changes that can be introduced into the m ouse genome using ES cells will also be discussed.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 21. Gene mapping; polymorphisms; disease genetic markers; marker assisted selection; gene expression; transgenes; non-convention, , 362–365, 1994
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