Abstract

The Booroola (FecB) locus has been mapped to sheep chromosome 6. M arkers flanking this gene were identified in sheep x ham ster somatic cell hybrids containing chromosomes 6 and 24. Partial genomic libraries were constructed from the cell lines, sheep-specific clones were selected from the libraries and microsatellite markers developed. Four markers mapped to sheep chromosome 6 and one marker mapped to sheep chromosome 24, demonstrating that chromosome-specific clones were generated from the hybrid cell lines and further defining the position of the FecB locus on sheep chromosome 6.
 

G. W Montgomery, J. M Penty, E. A Lord, H. M Henry, L. M Cambridge, T. E Broad

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 21. Gene mapping; polymorphisms; disease genetic markers; marker assisted selection; gene expression; transgenes; non-convention, , 17–20, 1994
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