Using PCR-RFLP method allelic polymorphism of kappa-casein gene and polymorphic variants of of DRB (exon 2) and DQB (5’- flanking region) loci were studied in different European bison populations. Allelic variants A and B of kappa-casein (specific for cattle) were found in European bison with frequencies P(A) = 22,4 % and P(B) = 77,6%. The tendency towards the loss of A allele was revealed as a result of high level of inbreeding. Only few polymorphic variants of DQB and DRB loci were found, some of them were unique for European bison. Phylogeny trees for European bisons populations and related species of Bovidae family were obtained"mine cluster analysis of the estimates of similarity for these loci. European bison populations demonstrates low level of differentiation in comparison with cattle breeds. The comparative analysis of DNA polymorphism of related animal groups is useful for genetic monitoring of rare species.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume 21. Gene mapping; polymorphisms; disease genetic markers; marker assisted selection; gene expression; transgenes; non-convention, , 147–150, 1994
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