Pouf1 is a 291 amino acid protein which binds to promoter of growth hormone, prolactin, beta-TSH and POUF1 genes and activates expression of these genes. As prolactin and growth hormone are essential for developing mammary system; POUF1 acts as a marker for genetic differentiation of milk production. Several researchers have reported that allele "A" is responsible for superiority of milk, protein or fat yields in cattle. The aim of this study was to estimate the allele frequencies in polymorphic site of exon six of POUF1 gene in three Iranian native and Holstein cattle. Genomic DNA was extracted from 3 Iranian native cattle breeds, including 97 Mazandarani, 87 Sarabi, 112 Golpaygani and 110 Holstein cattle. A 451 bp fragment of intron 5 and exon 6 were amplified and digested with HinfI restriction enzyme. Frequencies of allele "A" were 0.37, 0.27, 0.34 and 0.21 for Mazandarani, Sarabi, Golpaygani and Holstein cattle, respectively. Significant differences in genotype frequencies were found between Mazandarani and Golpaygani with Holstein cattle. No significant differences in genotype frequencies were found between Sarabi and Holstein cattle. Differences in allelic frequency between native Bos indicus breeds and Holstein at the present study might be due to differences in origin breeds, low number of samples, and/or as the effect of natural selection in native breeds.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume , , 22.61, 2006
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