In microarray experiments a whole genome-wide scan for differentially expressed genes is performed. Gene expression is measured indirectly by, for each gene printed on the array, measuring the fluorescence from labelled cDNA that hybridises to the spot(s) corresponding to this gene. Typically there are about 30 000 spots onto one array. The data analysis of these fluorescence-intensities is performed in three steps: (1) image analysis (recognising the spots), (2) normalisation of the data (correct for biases due to arraying technology), and (3) actual statistical analysis. The steps are described and discussed together with the design of microarray experiments. It is concluded that due to the large number of tests performed, and the perhaps more qualitative than quantitative nature of the results, follow up studies are essential to confirm and quantify the results.
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume , , 23.01, 2006
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