With the aim to investigate leptin genotypic variants, we have screened exon 3 of the bovine leptin gene by Denaturing High Performance Liquid Chromatography (DHPLC) in 147 animals of three Italian cattle breeds. We have also tested the sensitivity of DHPLC analysis in genotyping exon 3 of the leptin gene. Distinct DHPLC chromatograms were obtained and they were all examined by direct sequencing. Complete reproducibility of the observed chromatographic profiles was demonstrated. Here we show that DHPLC is an efficient technology for polymorphism investigation, allowing high-throughput genotyping without use of sequencing. Moreover a new allelic variation A to G was found located 152 bp from the start of the exon 3 that result in an Asn 99 Ser substitution.

L. Orro, F. Napolitano, G. Catillo, G. de Matteis, B. Moioli

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume , , 22.53, 2006
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