Ascaris suum is the largest intestinal parasite of pigs with higher prevalence in free range and organic production. Haemoglobin of A. suum origin, AsHb, is the most abundant protein in the pseudocoelomic fluid of adult worms and a major excretory⁄secretory protein of larva 3 (L3), L4 and adult worms. Immunization with AsHb resulted in increased reactivity to migratory stages in the liver; however, the porcine systemic response to A. suum is not entirely elucidated. Therefore, the current study aimed to investigate the interplay of proinflammatory and regulatory cytokines and host genetics to the AsHb stimulation in the peripheral blood. Accordingly, we used peripheral blood mononuclear cells (PBMCs) from clinically healthy, female, weaned piglets of five weeks old, and investigated the primary systemic response in terms of cell viability, phagocytosis and selective cytokine expression in naïve and LPS plus PHA costimulated PBMCs. Temporal expression patterns of IL-10, IL-6, IL-2, IFN-γ and TGF-β1 mRNA level for three time points (24, 48 and 72 hours) from two breeds (German Landrace, DL and Pietrain, Pi) were quantified using qRT-PCR. Phenotypic variation in cell viability between breeds was observed after 72 h of cultivation and only in the costimulated group. Significantly higher phagocytosis was observed in phagocytes of LR pigs compared to that of Pi. In naïve PBMCs treated with AsHb, significant breed effect was noticeable in case of IL-10, IL-6 and TGF-β1 expression, although the interrelationship between these regulatory cytokines was not always synchronous. In AsHb sensitized and co-stimulated PBMCs, the mRNA level of IL-2 was significantly affected by both breed and treatment at 24 h post culture. TGF-β1 was significantly upregulated in the costimulated Pi PBMCs where the significant effect of breed and treatment was noted. In conclusion, the systemic immune response to AsHb priming was characterized by a mixed Th1/Th2 profile of immune cytokines dominated by IL-10, IL-6 and IL-2 response. There was also substantial influence of the host breed indicating breed differences in the innate immune responsiveness to ascariasis. Key words: Ascaris suum, haemoglobin, innate immunity, cytokine, PBMCs, in vitro, pig, breed
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume Electronic Poster Session - Biology - Disease Resistance 3, , 607, 2018
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