Foetal and maternal placenta cells respond differently to a deleterious foetal mutation K. Rutkowska1, K. Flisikowski2, M. Lukaszewicz1& J. Oprzadek1 1Department of Animal Improvement, Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, 05-552 Jastrzebiec, Postepu 36a, Poland j.oprzadek@ighz.pl (corresponding author) 2Lehrstuhl für Biotechnologie der Nutztiere, Technische Universität München, Freising, Germany Foetal growth is a complex process that depends on balanced communication between foetus, placenta and a mother. Placental dysfunction is the main cause of the intrauterine growth restriction (IUGR). We previously identified a 3' deletion in the non-coding MIMT1 gene that, when inherited from the sire, causes IUGR and late abortion in Ayrshire cattle with variable levels of severity. The aim of this study was to analyse the expression of DDX1, RB1CC1, MKI67, EMP2, and AHNAK genes expressed in maternal and foetal placenta from 12 cows gestating MIMT1Del/WT foetuses and 12 cows gestating wild-type foetuses terminated on 94th ± 12 day of gestation. All foetuses were gestated in wild-type cows and all were fathered by the same MIMT1Del/WT sire. The relative gene expression difference between IUGR and wild-type in both tissues was calculated for each animal (ΔΔCt). All cDNA samples were assayed in triplicate and relative expression levels normalized to endogenous GAPDH expression. Two-way ANOVA was used to estimate the effect of interaction of the MIMT1 genotype with placenta tissues. The study revealed increased DDX1, RB1CC1, MKI67, AHNAK gene expression in the maternal placenta of the MIMT1Del/WT foetuses. No expression differences in foetal placenta for the studied genes were found. Our study evidenced that maternal and foetal placental cells respond differently to the foetal IUGR genotype. Caution is therefore warranted when analysing gene expression changes in non-dissected samples of placenta. The study was funded by the National Science Centre of Poland, research project no. 2016/23/N/NZ9/00232. keywords: placenta, cattle, gene expression, intrauterine growth restriction

Karolina Rutkowska, Krzysztof Flisikowski, Marek Lukaszewicz, Jolanta Oprzadek

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume Electronic Poster Session - Biology - Reproduction 1, , 76, 2018
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