Genetic variants such as single nucleotide variations (SNVs) and insertions/deletions (InDels) are present throughout the cattle genome. We recently published SNVs and InDels discovered through whole-genome re-sequencing of Gir, Girolando, and Holstein cattle and revealed variants associated with the olfactory transduction pathway, which may be related to residual feed intake (RFI). The aim of this study was to further analyze that data by focusing on gene-Transcription Factor (TF) binding sites. Genes presenting SNVs/InDels in TF binding sites were used to search for TF related to feed intake that were used to construct a gene-TF network for RFI across the three dairy cattle breeds. That gene-TF network highlighted genes (e.g. TRIM40, TBC1D7, and EPN3) and TF (e.g. STAT1 and MAX) that might have a role in explaining variation in residual feed intake. The Gir and Girolando breeds showed more enriched genes in the RFI gene-TF network suggesting that these breeds may have higher genetic variability for this trait. Even in Girolando animals (5/8 Holstein x 3/8 Gir), the contribution of Gir variants was over-represented at TF binding sites. Keywords: causal variants, functional analyses, next-generation sequencing,

Lucas Lima Verardo, Nedenia Stafuzza, Danisio Munari, Adhemar Zerlotini, Tatiane Chud, Dorian Garrick, John Cole, Joao Claudio Panetto, Marco Antonio Machado, Marta Martins, Marcos Vinicius Silva

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume Biology & Species - Bovine (dairy) 1, , 193, 2018
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