Abstract

Proceedings of the World Congress on Genetics Applied to Livestock Production, 11.892 Genome-wide distribution of allele-specific expression in Nelore steers muscle M. M. Souza1, A. Zerlotini2, S.C.M. Niciura1, M.I.P. Rocha, W.J.S. Diniz3, P.C. Tizioto4, P.S.N. Oliveira1, A.O. Lima3, J. Afonso3, A.S.M. Cesar2, L.L. Coutinho2 & L. C. A. Regitano1 1 Embrapa Pecuária Sudeste, Washington Luiz Highway, Km 234, 13560-970, São Carlos, Brazil mm.rissato@gmail.com (Corresponding Author) 2 Embrapa Informática Agropecuária, AV. Dr. André Tosello, 209 – Cidade Universitária, Campinas, 13083-886, São Paulo, Brazil 3 Federal University of São Carlos - UFSCar, Department of Genetic and Evolution, São Carlos, Washington Luiz Highway, Km 235, 13565-905, São Paulo Brazil 4 University of São Paulo - ESALQ, Department of Animal Science, Pádua Dias Ave., Piracicaba, 13418-900, São Paulo, Brazil The difference between the allelic expression of a gene is known as allele-specific expression (ASE), and it is a common event in mammal’s transcriptome. ASE is named genomic imprinting when guided by epigenetics mechanisms regarding the allele parental origin. Although these events are an important sources of phenotypic variation, knowledge thereof regarding cattle and effects on economically important traits are still incipient. Therefore, analysis and characterization of ASE profile in the muscle of adult Nelore animals were performed, and besides that, the ASE pattern and gene imprinting occurrence were checked in genes previously associated with meat tenderness. For this, RNA sequencing data of 146 Nelore steer muscles were used, and these animals were also genotyped for approximately 770,000 SNPs using Illumina BovineHD BeadChip. For each animal, a diploid genome was built based on their genotypes, and afterward, RNA-Seq reads were mapped to this genome. After counting the reads considering each SNP allele, a binomial test was applied to identify whether the allele contribution to total expression significantly diverged from 50%, followed by false discovery rate (FDR) test (0.05). Among 519 analyzed SNPs, 402 showed at least one sample with ASE, and they were spread among all autosomal chromosomes. SNPs showing ASE in more than five samples were tested regarding ASE extension, and most presented an incomplete pattern. Four ASE genes were previously identified in a Genome-Wide Association Study (GWAS) and five were found differentially expressed between extreme phenotype animals for meat tenderness. Therefore, the results will complement the bovine imprinted genes database, further they can provide new information to genetic effects prediction in animal breeding increasing the accuracy of meat tenderness selection. Keywords: allelic, ASE, gene expression, sequencing, transcriptome

Marcela Maria De Souza, Adhemar Zerlotini, Marina Ibelli Per Rocha, Simone Cristina M Niciura, Wellison Jarles da Diniz, Polyana Cristine Tizioto, Priscila Silva Neub de Oliveira, Luiz Lehmann Coutinho, Luciana Correia de Regitano

Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume Electronic Poster Session - Species - Bovine (beef) 1, , 892, 2018
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