Variation in ovine RAS Guanyl Releasing Protein 1 (RASGRP1) gene and its association with flystrike in New Zealand sheep L. E. R. Burrows1, H. Zhou1, C. M. A. Frampton3, R. H. J. Forrest2, J. G. H. Hickford1 1 Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, PO Box 85084, Lincoln University, Lincoln 7647, Christchurch, New Zealand 2 Faculty of Education Humanities and Health Science, Eastern Institute of Technology, Private Bag 1201, Napier 4142, New Zealand 3Department of Medicine, University of Otago, Christchurch 8140, New Zealand Flystrike is a parasitic disease that results in major cost to the sheep industry both nationally and worldwide. In recent years, research has focused on breeding sheep that are less susceptible to flystrike. Previous research has identified the RAS Guanyl Releasing Protein 1 gene (RASGRP1) as a possible candidate gene for flystrike resistance. To investigate this candidate gene, 791 blood samples were collected over five years from sheep with and without flystrike (n =421 with and n = 370 without), and of a variety of breeds. DNA from these blood samples was subjected to Polymerase Chain Reaction – Single Strand Conformational Polymorphism (PCR-SSCP) analysis using primers that targeted exon 16 of RASGRP1. Three variant sequences (A-C) were found and the presence of A was revealed to influence flystrike susceptibility (P = 0.015), while the presence of C was associated with a decrease in flystrike (P = 0.047). Keywords: flystrike, RASGRP1, sheep
Proceedings of the World Congress on Genetics Applied to Livestock Production, Volume Biology - Disease Resistance 2, , 641, 2018
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